Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience
Article Title: Developmental Nicotine Exposure Alters AMPA Neurotransmission in the Hypoglossal Motor Nucleus and Pre-B?tzinger Complex of Neonatal Rats
doi: 10.1523/JNEUROSCI.3711-12.2013
Figure Lengend Snippet: XIIn activity after AMPA microinjection into the XII motor nucleus. Representative traces of XIIn activity in a rhythmic brainstem slice before and after AMPA microinjection into the XIIMN. A, B, Raw (bottom) and integrated (top) traces from both control and DNE preparations before and after microinjection of 1 μM AMPA into the XIIMN (arrow indicates injection start time). Boxed regions in A and B are expanded in C and D, respectively. E, Tonic activity amplitude, calculated as percentage change from the preinjection baseline for control and DNE preparations (control, 483.36 ± 119.84%; DNE, 658.11 ± 180.43%; p = 0.484). The amplitude of tonic activity was measured in the periods between phasic XIIn bursts. F, Duration of increased tonic activity after AMPA injection in control and DNE preparations (control, 14.03 ± 1.68 s; DNE, 40.18 ± 7.43 s; p = 0.012). Error bars represent SEM.
Article Snippet: Microinjection of AMPA Thick-walled borosilicate glass capillary tubes (OD 1.5 mm, ID 0.75 mm, Sutter Instruments) were pulled to tip sizes ~1 μ m in diameter (resistance, 1.2–3.8 MΩ).
Techniques: Activity Assay, Microinjection, Control, Injection